Construction of luciferase reporter bacteriophage A511::luxAB for rapid and sensitive detection of viable Listeria cells
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چکیده
منابع مشابه
Evaluation of luciferase reporter bacteriophage A511::luxAB for detection of Listeria monocytogenes in contaminated foods.
A511::luxAB is a recombinant derivative of a broad-host-range bacteriophage specific for the genus Listeria, transducing bacterial bioluminescence into infected cells. In this study, we have evaluated its use for rapid and easy testing of contaminated foods and environmental samples for the presence of viable Listeria cells, in comparison to the standard plating procedure. With a short preenric...
متن کامل“ Evaluation of Luciferase Reporter Bacteriophage A
Liu et al. Journal of Microbiologucal Methods, 51, 369-377, 2002.* Ley et al. Can J Microbiol. 39, 821-825, 1993.* Kono, Advanced Drug Delivery Reviews, 53 307-319, 2001.* “Alteration of Tail Fiber Protein gp38 Enables T2 Phage to Infect Escherichia coli 0157:H7” By: Masatoshi Yoichi, Michiham Abe, Kazuhiko Miyanaga, Hajime Unno, Yasunori Tanji, 2005. “Genome Plasticity in the Distal Tail Fiber...
متن کامل“ Evaluation of Luciferase Reporter Bacteriophage A 5 11 : : luxAB for Detection of Listeria monocytogenes in Contaminated Foods
Liu et al. Journal of Microbiologucal Methods, 51, 369-377, 2002.* Ley et al. Can J Microbiol. 39, 821-825, 1993.* Kono, Advanced Drug Delivery Reviews, 53 307-319, 2001.* “Alteration of Tail Fiber Protein gp38 Enables T2 Phage to Infect Escherichia coli 0157:H7” By: Masatoshi Yoichi, Michiham Abe, Kazuhiko Miyanaga, Hajime Unno, Yasunori Tanji, 2005. “Genome Plasticity in the Distal Tail Fiber...
متن کاملSensitive rapid detection method for viable bacterial cells.
A rapid sensitive method for the detection of viable bacterial cells is described in which P as inorganic orthophosphate is used to label the cells. Factors affecting the uptake of P by cells as well as the sensitivity of the method have been explored with suspensions of Aerobacter aerogenes. The uptake of PO(4) is dependent on several factors. Of various incubation media tested, one composed o...
متن کاملSensitive detection of viable Listeria monocytogenes by reverse transcription-PCR.
Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of three L. monocytogenes genes, iap, hly, and prfA, was examined to determine a...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1996
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.62.4.1133-1140.1996